Welcome
An Introduction to Agar - What it is and How to Work With It
An Introduction
Agar petri dishes provide the containment for bacteria while the components of Agar provide the medium for colonies to spread and grow; you can continue -or begin- reading prior here on psilocybin growth, https://hypnochain.com/hive-163105/@trezzahn/mycelial-spore-utilization-on-agar-petri-dishes-p2-4-in-a-two-blog-series and https://hypnochain.com/hive-163105/@trezzahn/an-introduction-to-spore-growth-and-reproduction-in-a-controlled-environment-p1-4-of-in-a-two-blog-series .
More information can be found here beginning this journey of growth https://hypnochain.com/@trezzahn
Agar Agar
There are three reagents needed to mix this Agar medium -
They can very easily be obtained at health food stores, an organic supermarket, or ordered and consist of the following:
1 of: - [Preferably] Brewers Yeast Powder,
Or, General Nutritional Cooking Yeast
Balt Marley Extract
Agar Agar, or of other means for medium if another tek rather this one be utilized.
Starting Off
Before beginning, ensure ample circulation is available for transfer of mixture. Initially, mix all the reagents in the appropriate proportions and amounts in a sanitized and sterilized mixing jar and spoon -
per 3 small 2in x 2in petri dishes:
86mL Hot Tap Water
0.172g Decided Upon Yeast Extract
1.715g Malt Extract Agar
1.715g Agar Agar
Beginning Work
Wrap aluminum foil around the top of a measuring cup to get a frame for when after the Agar mixture is added to reduce the amount of air contaminant that could enter, all while utilizing a fume hood, or if not available, consistent circulation of air.
Pour the Agar mixture from the mixing jar to the measuring cup while utilizing air circulation and minimal time where aluminum foil does not cover the top, and immediately cover after poured.
Place the now covered measuring cup with the Agar into a pressure cooker or closed air-tight pot and ensure the aluminum foil is tight around the top.
The Process
Over a low-medium stove-top, bring the large pot or cooker to a boil, ensuring no air pressure escape and that no spillover sounds are heard from within the pot as the covered measuring cup with Agar and water interact. Allow the pot to stay at low boil for one hour, then put low simmer for one hour, and cool for 15 minutes on burner.
Once the pouring station has been effectively set up, sterilized, and sanitized, the cooker can be released or pot, opened, releasing the pressure from inside.
If completed correctly with an appropriately sized measuring cup, pouring from the spout will be simple if no spillover has occurred. Lift the cup out safely, with an oven mitt if necessary, and place in the SAB, or begin immediately if none.
Lift the top of each dish, pouring in a slow circular motion, as per recent posting on pouring technique, while ensuring the bottom of the dish fills, while having no physical contact with any dishes.
Close each dish immediately after with the other hand and continue to pour the required number of dishes.
Ending Off
Seal each dish with parafilm or masking tape and store in the fridge or a cool place for use with the acquired spores, or elsewhere if to be used in the near future/immediately.
Once cooled, dishes can br stored facing down so any condensation that may develop accidentally would not affect the medium in the future. See below:
In Conclusion
Hopefully this was a knowledgeable posting for this section of my blog. Stay tuned as we continue to dive deeper in depth, examining all key components of mycellial growth!
This post may be of interest to those interested in mental health relatability: @trezzahn/a-cross-examination-of-the
Updated Links (for Ecency {+app} and PeakD)
Ecency
Starting Out, and Psychoactive Research
@trezzahn/psychoactive-research
or
@trezzahn/psychoactive-research
Medical Benefits - Writing (Articulate)
@trezzahn/a-cross-examination-of-the
or
@trezzahn/a-cross-examination-of-the
Outline Postings
HypnoChain Posting
@trezzahn/general-hypnochain-outline-month-following
or
@trezzahn/general-hypnochain-outline-month-following
StemGeeks Posting
@trezzahn/petri-dish-technique-and-methodology-month-following
or
@trezzahn/petri-dish-technique-and-methodology-month-following
Introduction to Psilocybin
HypnoChain Posting
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or
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or
@trezzahn/psilocybe-mycelial-and-their-colonization-on-agar-agar
Section 1
Part 1.1
@trezzahn/an-introduction-to-spore-growth-and-reproduction-in-a-controlled-environment-p1-4-of-in-a-two-blog-series
or
@trezzahn/an-introduction-to-spore-growth-and-reproduction-in-a-controlled-environment-p1-4-of-in-a-two-blog-series
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or
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or
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Section 2
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or
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or
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or
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or
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or
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or
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PeakD
Starting Out
@trezzahn/starting-out-future-work
Psychoactive Research
@trezzahn/psychoactive-research
Medical Benefits - Writing (Articulate)
@trezzahn/a-cross-examination-of-the
Outline Postings
HypnoChain Posting
@trezzahn/general-hypnochain-outline-month-following
StemGeeks Posting
@trezzahn/petri-dish-technique-and-methodology-month-following
Introduction to Psilocybin
HypnoChain Posting
@trezzahn/3f3eyz-psilocybe-mycelial-and-their-colonization-on-agar-agar
StemGeeks Posting
@trezzahn/psilocybe-mycelial-and-their-colonization-on-agar-agar
Section 1
Part 1.1
@trezzahn/an-introduction-to-spore-growth-and-reproduction-in-a-controlled-environment-p1-4-of-in-a-two-blog-series
Part 1.2
@trezzahn/an-introduction-to-petri-dish-preparation-for-use-with-growth-medium-day-two-p1-4-in-a-two-blog-series
Part 2.1
@trezzahn/mycelial-spore-utilization-on-agar-petri-dishes-p2-4-in-a-two-blog-series
Part 2.2
@trezzahn/an-introduction-to-agar-and-its-utilization-working-with-it-proper-technique-and-further-use-p2-4-in-a-two-blog-series
Part 3.1
@trezzahn/liquid-culture-what-it-is-methodology-and-colonization-and-further-work
Part 3.2
@trezzahn/mycelium-growth-preparation-agar-additionals-general-timeframes-and-further-work-the-fun-part
Part 4.1
@trezzahn/mycelial-storage-syringes-cold-storing-and-other-methods-p4-4-of-a-two-blog-series
Part 4.2
@trezzahn/agar-petri-dish-methodology-technique-and-where-we-re-off-to-next-p4-4-of-a-two-blog-series
Section 2
Part 1.1
@trezzahn/mycelial-caking-and-growth-an-introduction-to-colonization-mediums-a-continuation-of-section-1-section-s-2-p1-1-4-in-a-two-blog
Part 1.2
@trezzahn/brown-rice-flour-and-rye-grain-substrate-a-scientific-examination-of-its-utilization-as-a-medium-for-mycelial-growth-a
Part 2.1
@trezzahn/mycelial-consolidation-and-colonization-of-a-substrate-medium-a-continuation-to-colonization-mediums-cont-section-1-section-s-2
Part 2.2
@trezzahn/shotgun-fruiting-chamber-s-construction-use-and-utilization-a-continuation-of-section-2-p2-2-4-in-a-two-blog-series
Part 3.1
@trezzahn/birthing-of-consolidated-mycelium-cakes-pailocybin-growth-a-continuation-of-section-2-p3-4-in-a-two-blog-series
Part 3.2
@trezzahn/fruiting-and-growth-of-birthed-mycelium-cake-psilocybin-growth-a-continuation-of-section-2-p3-4-in-a-two-blog-series
Part 4
@trezzahn/harvest-and-flush-of-birthed-mycelium-cake-substrate-psilocybin-growth-a-conclusion-of-section-2-p4-4-in-a-two-blog-series
my personal blog:
and research blog:
PeakD:
hive.buzz + hive.d.blog:
https://d.buzz/#/@trezzahn/t/buzz?ref=nav
Thanks again all for hopefully some potential interest!
Best regards,
- Trezzahn
Psychoactive Research: https://hypnochain.com/steemgeeks/@trezzahn/psychoactive-research
Old Links
You can see next weeks posting on liquid culture here:
@trezzahn/liquid-culture-what-it-is-methodology-and-colonization-and-further-work
Or
The examination of spore utilization from growth of mycelium on HypnoChaiin Monday is inspected here:
Or
@trezzahn/mycelial-spore-utilization-on-agar-petri-dishes-p2-4-in-a-two-blog-series
Stemgeeks @
You can learn how to grow Agar, and how to prepare your dishes
here:
Or
Hypnochain @
https://hypnochain.com/@trezzahn
Where you can see first weeks posting on Agar growth and utilization through wedging and other techniques,
here:
Or
my personal blog:
and research blog:
PeakD:
hive.buzz + hive.d.blog:
https://d.buzz/#/@trezzahn/t/buzz?ref=nav
Thanks again all for hopefully some potential interest!
Best regards,
- Trezzahn
Psychoactive Research: https://hypnochain.com/steemgeeks/@trezzahn/psychoactive-research
#mushroom #psilocybin #psilocybe #mycelium #mycelial #petri #spore #psychedelic #psychoactive #hypnotic #hive #ecency #hypnochain #stem #stemgeeks #cc