The sun hits your skin and converts 7-dehydrocholesterol into pre-vitamin D3. Liver converts pre-D3 into 25-hydroxy-vitamin D3. Kidneys convert that into 1,25-dihydroxy D3.
The last step is tightly regulated so 1,25-dihydroxy D3 levels don't budge until you get severely deficient, so they usually measure 25-hydroxy D3 instead. This reflects total diet and endogenous vitamin D.
That's all for now!
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