Chromatography could be a physical technique for separation supported the variations within the affinity of the compounds being analyzed against 2 phases, stationary / stationary part and automobile / mobile part.
Thus, the mixture of compounds will bear surface assimilation and method|natural action|action|activity} by introduce succession in order that the mobile part can consecutive dissolve the compounds in succession and therefore the separation process will occur as a result of the mixture of compounds has completely different solubility between the 2 phases.
The stationary part employed in natural action will be either solid or liquid. silicon dioxide and corundum area unit samples of solids that area unit typically used as stationary phases because of their ability to sorb materials to be separated (as adsorbents).
Both will be used as column fillers in gravity chromatography, high natural action and conjointly as a skinny layer maker for TLC. The stationary part may be a liquid with a support introduce the shape of a solid. One example is in chromatography wherever the stationary part is water that could be adsorbed by polyose molecules on paper (paper is a support phase), whereas within the natural action the stationary part is solid that's connected to the capillary that is stable to temperature and this stationary part has constant pore and size.
The mobile part will be a gas in gas natural action, it may be a liquid as in chromatography, skinny layer natural action or chromatography. If the automobile part could be a gas, it's known as a gas vector / carrier gas, whereas if it's a liquid it's known as eluent / solvent.