Thiamine Mononitrate (X2)
Potassium Citrate (X2)
Magnesium Chloride (X1)
Sodium Bicarbonate (X1)

X=Tablespoon
12 Ounce of Water

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sipped about a shot glass over the night, well that was intense.

and it is still doing things, not very good for sleeping.

this is the most effective hodgepodge combination tried so far.

its break down the acids from my legs to head, effective almost instantly, and it lasts for hours.

tested megadose of Thiamine for months, to get a good idea what it does, mixing it with buffered alkaline minerals seems to multiply the binding properties.

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https://www.reddit.com/r/LongCovid/comments/yl3a8b/thiamine_my_favorite_b_vitamin/

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Cytosine-Guanine Dinucleotides (CpGs)

Methylated Cytosine (5mC)

5-Carbon Cytosine
5-Methylcytosine

HLA-DR (gene)

https://en.m.wikipedia.org/wiki/HLA-DR

Gene expression and epigenetic markers of prion diseases

https://link.springer.com/article/10.1007/s00441-022-03603-2

Methylation predominantly occurs at the 5’-carbon of cytosine in cytosine-guanine dinucleotides (CpGs) which are unevenly distributed across the genome being concentrated in “islands” near to gene regulatory regions. Methylated cytosine (5mC) is sometimes referred to as the 5th base of DNA.

DNA Methylation:

X-Chromosome
DNMT3A / DNMT3B
DNMT (MTase domain)
Carboxy Terminus
Chromatin Reading Domains

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"Methylation predominantly occurs at the 5’-carbon"

Thiamine: Thiazole

is that the exact location of Prion misfolding & what Thiamine interacts with?

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Thiazole
Aminothiazole

Thiazole: A Versatile Standalone Moiety Contributing to the Development of Various Drugs and Biologically Active Agents

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268695/

Though free thiazole cannot be spotted in nature, the ring of thiazole is detected in several natural compounds, such as peptide alkaloids, metabolites, cyclopeptides (and Thiamine).

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Sodium and Potassium Ions in Proteins and Enzyme Catalysis

https://pubmed.ncbi.nlm.nih.gov/26860304/

Na(+) and K(+) play important roles other than being nonspecific ionic buffering agents or mediators of solute exchange and transport. Molecular evolution and regulated high intracellular and extracellular M(+) concentrations led to incorporation of selective Na(+) and K(+) binding sites into enzymes to stabilize catalytic intermediates or to provide optimal positioning of substrates. The mechanism of M(+) activation, as derived from kinetic studies along with structural analysis, has led to the classification of cofactor-like (type I) or allosteric effector (type II) activated enzymes.

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pH-Dependent
Thiazole

Effect of pH and concentration on the chemical stability and reaction kinetics of thiamine mononitrate and thiamine chloride hydrochloride in solution

https://bmcchem.biomedcentral.com/articles/10.1186/s13065-021-00773-y

Thiamine Assays—Advances, Challenges, and Caveats

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390807/

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Thiazole
Thiazolium Salts (Cation) Alkaline

Thiazolium Salts as Chalcogen Bond Donors

https://pubs.acs.org/doi/10.1021/acs.cgd.2c00510

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https://en.m.wikipedia.org/wiki/Benzothiazole

https://en.m.wikipedia.org/wiki/2-Aminothiophenol

https://en.m.wikipedia.org/wiki/Benzoxazole

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Tafamidis

https://en.m.wikipedia.org/wiki/Tafamidis

Tafamidis, sold under the brand names Vyndaqel and Vyndamax, is a medication used to delay disease progression in adults with certain forms of transthyretin amyloidosis. It can be used to treat both hereditary forms, familial amyloid cardiomyopathy and familial amyloid polyneuropathy, as well as wild-type transthyretin amyloidosis, which formerly was called senile systemic amyloidosis. It works by stabilizing the quaternary structure of the protein transthyretin. In people with transthyretin amyloidosis, transthyretin falls apart and forms clumps called (amyloid) that harm tissues including nerves and the heart.

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Colchicine

https://en.m.wikipedia.org/wiki/Colchicine

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apparently alkaline Ph alters the structure of Thiamin & Thiazole, into an oxidative state, making something more like Tafamidis.

if my Alkaline Thiamine brew is anything like Tafamidis, this paper is talking about 20 mg, and im taking more like 2000+ mg.

high dose gives a hint of a headache, but my joints seem to be way more flexible, a bit of ear ringing, but goes away after a few days of stopping.

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Long-Term Survival With Tafamidis in Patients With Transthyretin Amyloid Cardiomyopathy

https://www.ahajournals.org/doi/10.1161/CIRCHEARTFAILURE.120.008193

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https://en.m.wikipedia.org/wiki/Transthyretin

Transthyretin:
Role in disease:
Tafamidis

this link talks about Tafamidis as well, my interest is regarding it having the Thiazole identical to Thiamine.

found information regarding high dose of Nician (B3) may have a negative Methylation binding effect, and Thiamine (B3) actually reverses it.

in my many experiments, lt was bringing down Ph with Citric Acid that converted Thiamine into a completely different compound with a strong Sulfur smell, yet the one paper says its Alkaline high Ph that makes Thiamine unstable.

so my experience is the complete opposite, but its better to just use Buffered minerals with a balanced Ph, to act as coenzyme activators.

all im doing is taking regular B1 with baking soda (alkaline), potassium citrate (for kidney stones), magnesium chloride (constipation).

the most basic & inexpensive forms of each nutritional supplement, nothing fancy, its just when combined with B1 triggers enzymes in mitochondria.

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Thiazole
Enzyme
THI1
2-Ring Octamer
Histone H2A

https://en.m.wikipedia.org/wiki/Histone_H2A

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Histone H5 is a potent Antimicrobial Agent and a template for novel Antimicrobial Peptides

https://www.nature.com/articles/s41598-018-20912-1

trick is this 5-Carbon, and looking at Histone H2A found this one being used as a full spectrum antibiotic.

Histone H5

(is it from 5-Carbon metabolite or pathway?)

5-5arbon
Pentose
Deoxyribose
Ribonucleotide Reductase
Thymidylate Kinase

https://en.m.wikipedia.org/wiki/Pentose

https://en.m.wikipedia.org/wiki/Deoxyribose

https://en.m.wikipedia.org/wiki/Ribonucleotide_reductase

https://en.m.wikipedia.org/wiki/Thymidylate_kinase

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Genetic prion disease–related mutation E196K displays a novel amyloid fibril structure revealed by cryo-EM

https://www.science.org/doi/10.1126/sciadv.abg9676

Each subunit forms five β strands stabilized by a disulfide bond and an unusual hydrophilic cavity stabilized by a salt bridge. Four pairs of amino acids from opposing subunits form four salt bridges to stabilize the zigzag interface of the two protofibrils.