So today was alot easier and straight forward than yesterday in every sense of it. I got to the laboratory at around 9:35 but I signed in 8:15am (Corrupt practices) but everyone understood the drill perfectly.
I headed straight down to the chiller and got my 40 Pure MRS plates and set them on my workbench like a boss. Opeyemi was such a darling today,even without me telling her, Babe took my plates and went to dry them in oven. She was such a darling!.
And yes, test-tube rack almost had me off balance today as every one was already busy with their racks and none was available for lease. So here I was with my yoghurt samples and Petri dishes and yet to test-tube rack for my test-tube of ringer solution. Just out of the blue, Emmanuel offered me his test-tube rack and he helped me in the serial dilution process. I really wasn't expecting it because we weren't exactly close or anything. I kind of expected the help Opeyemi was offering me but not the one from Emmanuel.
So yes Emmanuel helped me to serially dilute me sample.
My test-tube was not as big as 10ml so I had to use about 3ml in totality running at 2.7ml of Ringer solution and 0.3ml of the yoghurt sample working on 10:1000000 dilution factor i.e 10^5 factor. Emmanuel helped me with all, though not as if I was entirely useless in the whole process, I should put it as he assisted me as in we walked together as in he made my work faster.
Yes after my serial dilution of which I was mute all through the process so as not to introduce contaminants into my innoculum I had my victory dance.
And yes I went on to spread plate method of innoculating with the amiable assistance of Olamide. You know today, I felt like God was actually trying to prove a point to me crystal clear " I would go before you and make every crooked way straight" and trust me it is a straight road. We inoculated 40 plates ofcourse taking the factor 3 and factor 5 of the serial dilution and plating them in duplicate.
So yes I was to incubate my plates anaerobically that is in the absence of air so I was given an improvised anaerobic jar. I was given a canister which I would put a candle in and cover it up till the candle burns out in the absence of oxygen then my plates in it would be incubated at 37°C.
That was all with laboratory today.
After Laboratory events.
