Polymerase Chain Reaction developed by Kary Mullis in 1980s, was one of the greatest invention of the past century.....
It targets a segment of a gene and amplifies into millions of copies, it is also time and temperature dependent.
Steps involves
Denaturation of the double stranded DNA at 94 Degree Celsius
-Annealing of the primer to template at 55 Degree Celsius
-Extension of the target sequence at 72 Degree Celsius
PCR reagents involve
-Primers
-Buffers
-Taq polymerase
-Forward and Reverse primers
-PCR grade water
