Vault (Organelle)

https://en.m.wikipedia.org/wiki/Vault_(organelle)

vaults (especially the MVP) were over-expressed in cancer patients who were diagnosed with multidrug resistance.

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Macromolecule

Macromolecular Crystal Structures

Phospholipid-induced prion conversion

Macromolecules co-purified with PrPSc from brain tissues were suspected to be involved in PrP conversion. One of the main findings was that polyanionic compounds, such as RNA and proteoglycans, interact and convert endogenous and bacterially expressed PrPC.

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Glycosyl
Glycosylation
Glycoprotein
DNA Glycation
Advanced Glycation End-products (AGEs)
Methylglyoxal
Glyoxal
Proteoglycan
Polyanion
Polyanionic Electrolyte
Glycosylphosphatidylinositol (GPI)
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Glycosylation is the process by which a carbohydrate is attached to a target macromolecule, typically proteins and lipids.

prion protein sensitizes neurons to glutamate-induced excitotoxicity.

interaction between metabotropic.glutamate receptor 5 and cellular prion protein.

Among the viral proteins is HIV-1 envelope Glycoprotein 120

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Neurometals in the Pathogenesis of Prion Diseases

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866166/

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this article may be a bit misleading, by saying Prion "uses" Zinc & Copper for spreading.

because brass is toxic to prion, what its likely doing is breaking the neurological metal connections down into deposits.

testing minerals, vinegar & niacin, it never occurred to me that Methyl Doner as Osmolyte & Inorganic Ions work together for homeostasis, acting like Brass & Methylene Blue.

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Glucose
Glutamate
Melanin

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Glucose metabolism regulates expression of hair-inductive genes of dermal papilla spheres via histone acetylation

https://www.nature.com/articles/s41598-020-61824-3

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TMG
Trimethylglycine
Betaine Anhydrous
Glycerol+Nitrogen

IHN
Inositol Hexanicotinate
Nicotinate Inositol
Nitrogen+Glycerol

for a Sulfur element Thiamine B1, NAC, or just MSM.

but most important is the Inorganic Salts Ions with Nitrogen & Glycerol.

plus Acetate seems important as well, to charge the Ions.

NAC (N-acetylcysteine)

Acetyl = Acetate = Acetic
Acetyl-CoA = Vinegar

testing both, the supplements together & the raw material, vinegar, vegetable glycerin, minerals complex, nitrogen source.

its ultimately doing rhe same thing, the supplements are overlapping elements.

its ultimately looking like Creatinine.

problem with Creatinine is its found is excess with Kidney Disease,

Nephrotic Syndrome
Nephritic Syndrome

the problem is not from the Creatinine, but Retinol disfunction that locks all other Kidney Enzyme functions called Hypervitaminosis A.

what Creatinine seems to be missing is the Glycerol & Salt Ions, and possibly the Acetate Acetyl.

its a complex supplement, so it difficult to tell what its missing.

but ultimately the goal is to fix the Retinol pathways, before anything else begins to improve.

if i had to make a guess what fixes Retinol lock, its the Vinegar, Minerals & Glycerol.

this combinations is so powerful, it absorbs threw the skin instantly, identical to DMSO.

Nitrogen element is more important for different things down the enzyme pathways.

may have discovered a way to manufacture something close enough to Vegetable Glycerin, without having to do it via Soap Making using Oil & Lye.

vinegar with live yeast (mother), just slowly keep adding sugarnor honey, as it continues to ferment, eventually it will become oversaturated, and that covers both Vinegar (Acetyl) & Sugar Alcohol (Polyol).

its close enough to Glycerol to do the thing, just needs a tablespoon of Minerals.

regarding Hypervitaminosis A, dont think is because Vitamin A is toxic, but pesticides & parasites manufacturing identical chemical compounds as pesticides.

all chronic disease seems to be dependent upon this as the first cog in metabolism & homeostasis.

the biggest clues right now..

regarding the Nitrogen element in both TMG & IHN, is that they dont actually cure any disease.

but they do have something in common, called Methyl Doner, a Glycerol that is also a Osmolyte.

this is the biggest clue into how Hydrogen is the secret element.

Nitrogen is helpful regarding NAD/NADH mitochondria metabolism.

to loosen a Prion bond, is the goal here.

Inorganic Salts & Osmolyte work together almost as a single unit, and both are charged via Acetate (Vinegar).

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Betaine (Trimethylglycine), in my opinion, combined with Vinegar & Minerals, its the backbone for all the B Vitamins, Amino Acids, Lipids, Creatinine, SAMe, Glutathione ect..

and its the smallest & likely most effective Osmolyte compound, known to refold misfolded proteins, or Prion Amyloidosis.

Homocysteine MTHFR disfunction is kind likebthe very first step in toxic enzyme locks, it fixes that to.

so im assuming it repair Retinol pathways as well.

its so small it puts Hydrogen into the toxic acids, breaks everything up at the core, just like the plan all along, Enzyme Activator, Osmolyte, Methyl Doner ect..

im trying to pump the vinegar fermentation with raw honey, oversaturate the sugars to see if it becomes like a Glycerol, and then would only require a Nitrogen & Minerals.

but thats down the road, not sure if that will actually work.

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N-Glycoprotein
Nitrogen (Osmolyte)

S-Glycoprotein
Sulfur (Spike)

Heparan Sulfate
N-Deacetylation
N-Sulfation

N-Acetylglucosamine
(GlcNAc)

Carrageenan (Red Algae)
Sulfated Polysaccharide
Galactopyranose
Anionic Polysaccharide

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its likely this is whis why Nitrogen is helpful with Polyol, to keep it from becoming sulfated.

may have figured why Nitrogen is so important, because it the only element to break apart a Sulphur bond.

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Cellular internalization of alpha-synuclein aggregates by cell surface heparan sulfate depends on aggregate conformation and cell type

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5566500/

Heparan sulfate has been found in all extracellular amyloid deposits investigated, regardless of the nature of the amyloid protein.

The sulfation code for propagation of neurodegeneration

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6036190/

It was reported for α-synuclein that internalization of amyloid fibrils depends on heparin sulfate, whereas that of smaller non-amyloid oligomers does not. Thus, binding of smaller oligomeric forms of tau and other protein aggregates to HSPGs should be investigated.

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Methylation
DNA Methyltransferase
Acetylation
Histone Acetyltransferase
Methyl
Acetate Ion
Enzyme
Chromatin
Osmolyte

The Key Role of DNA Methylation and Histone Acetylation in Epigenetics of Atherosclerosis

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7521974/

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The Genetic Systems of Mitochondria and Plastids

https://www.ncbi.nlm.nih.gov/books/NBK26924/

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Fenbendazole
Benzimidazole
Oxfendazole
Imidazole
Alkaloid
Tubulin Inhibitor
Mitotic Inhibitor
Benzimidazolide
Methoxycarbonyl
Phenylsulfanediyl

https://en.m.wikipedia.org/wiki/Imidazole

https://en.m.wikipedia.org/wiki/Benzimidazole

https://en.m.wikipedia.org/wiki/Oxfendazole

https://en.m.wikipedia.org/wiki/Mitotic_inhibitor

Fenbendazole
Benzimidazole

Fenbendazole is a member of the class of benzimidazoles.

Most prominent benzimidazole complex features N-ribosyl-dimethylbenzimidazole, as found in vitamin B12.

Benzimidazole and its derivatives as cancer therapeutics: The potential role from traditional to precision medicine

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9978992/

Benzimidazole contains a hydrogen atom attached to nitrogen in the 1-position and can form a tautomer upon interaction with aprotic solvents, such as water.

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Tautomers are structural isomers of chemical compounds that readily interconvert. The chemical reaction interconverting the two is called tautomerization. This conversion commonly results from the relocation of a hydrogen atom within the compound.

Ammeline
Annular Tautomerism

Role of tautomerism in RNA biochemistry

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4274630/

On Tautomerism and Amphoterism: An In-Depth Structural and Physicochemical Characterization of Ammeline and Some of Its Salts

https://onlinelibrary.wiley.com/doi/10.1002/anie.202404927

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Betaine Osmolyte Methyl Doner (Prion) & Fenbendazole Annular Tautomer Mitotic Inhibitor (Cancer).. both very specifically have to do with Hydrogen Bonds.

by adding honey to vinegar fermentation, does appear to be making a natural form of Glycerol, so that is promising.

but wonder about Betaine with drops of DMSO, to replicate a Fenbendazole like derivative, deactivate both Prion & Cancer?

mixed equil parts Betaine & MSM with water in an eye dropper, they dont mix together in water, sinks to the bottom, but the taste of it is exactly the strange chalky flavor of Fenbendazole.

MSM is very bitter, Betaine is very sweet, together tastes like chalk or almost like wood glue, its hard to explain.

it turns slick like silicone on the skin, but not oily.

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DMSO and betaine greatly improve amplification of GC-rich constructs in de novo synthesis

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2883997/

Though we found no benefit in employing either DMSO or betaine during the assembly steps, both additives greatly improved target product specificity and yield during PCR amplification.

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TMG (Betaine)
DMSP (DMSO)

i think the Betaine is turning the DMSO/MSM into the Osmolyte DMSP.

3 parts Nitrogen, to 1 part Sulfur, for Fenbendazole derivative.

and the Acetate Ions with oversaturated Honey Glycerol help to bind up parasites & cancer microtubules.

Betaine seems to neutralize the side effects of both Acetate Ions & DMSO Sulfur.

Acetyl is a Osmolyte very specific to brain homeostasis, found a bunch of articles.

Acetyl (Vinegar)
Acetyl-CoA
N-acetylaspartate (NAA)

beginning to get the idea that, if all the Osmolytes are combined together, then..

what do all Osmolytes have in common?

the most reduced non-toxic molecular state of Hydrogen, Oxygen, Carbon, Nitrogen & Sulfur.

see how difficult it was to just find out this simple observation, my previous months ago vinegar trial was just put everything in it & see what happens, now i know exactly what to refine the ingredients.

Vinegar
Betaine
MSM (not much)
Minerals

one important the reason for the minerals is because Osmolyte elements are identical to Chelation compounds, and would likely Chelate electrolytes out.

honey added seems very similar to Glucagon.

quickly looking at Glucagon, it has an odd reaction, temporary slows digestion?

this tells me that its almost like proto-amino acids, or almost like stem cell food, the body can instantly make anything it wants with it.

one thing vinegar, niacin derivative & sulfur, it dont take very long to know you took it, lightning fast.

my final test is to convert regular hit niacin into non-flush, vinegar along only enhances the flush, bit together with honey as a carbon buffer may act as a glycerol.

reason i think honey & vinegar can be used as a glycerol substitute, both honey & glycerol are sweet, vinegar is the byproduct of alcohol production, and glycerol is a sugar alcohol called polyol.

if this is correct, then it should buffer niacin flush, because the simple sugar one-carbon metabolic CH3 methyl doner.

problem with pure vegetable glycerol is that it wont actually mix or bind with anything else.

honey & vinegar together at first is a little sticky, over time it becomes almost oily like silicone.

for over 2 years i was testing vinegar minerals, with a concern Acetate Ions may be toxic Anion or Cation, just recently found enough evidence it is not toxic, but causes crystal like formations in the skin, making it dry & irritated nothing seemed to quite break it up, except the Betaine instantly started helping, so the honey vinegar idea came about, because combined with the nitrogen & a little sulfur, it chelated the nano salt crystals.

Acetate
Hot-Ice (Crystals)

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Acetate
Hot-Ice (Crystals)

https://pediaa.com/what-is-the-difference-between-sodium-acetate-and-sodium-acetate-trihydrate/

https://sciencenotes.org/make-hot-ice-from-baking-soda-and-vinegar/

Glucagon

https://en.m.wikipedia.org/wiki/Glucagon

Glucagon’s Metabolic Action in Health and Disease

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8513137/

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CHARM

Coupled Histone tail for Autoinhibition Release Methyltransferase

https://en.m.wikipedia.org/wiki/Histone_methyltransferase

https://en.m.wikipedia.org/wiki/Betaine%E2%80%94homocysteine_S-methyltransferase

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Astaxanthin

https://en.m.wikipedia.org/wiki/Astaxanthin