Genomic Insight into the Host–Endosymbiont Relationship of Endozoicomonas montiporae CL-33T with its Coral Host

https://www.frontiersin.org/articles/10.3389/fmicb.2016.00251/full

Key Word: (TIGR04523)

A Giant Secreted Protein of E. montiporae Might Modulate Host's Intracellular Vesicle Trafficking

To survive inside a host cell, intracellular pathogens and symbionts have to evade the host defense system, which relies on intracellular vesicle trafficking.

In the present study, it was determined that E. montiporae contained at least one gene that may be essential for this purpose. In that regard, EZMO1_3398 is a giant extracellular protein-coding gene, which encodes 3872 amino acids and comprises the myosin tail (pfam01576) and Mycoplasma helix-rich (TIGR04523) domains at its N-terminus.

Furthermore, EZMO1_3398 was predicted as a Sec-dependent secreted protein but not a membrane protein. The myosin tail domain contains α-helices that can form a coiled-coil structure for dimerization.

Regarding the Mycoplasma helix-rich domain, it is still functionally unclear, although it is common in Mycoplasma species.

Based on structure prediction, EZMO1_3398 could be a chimeric protein, encompassing bacterial and eukaryotic protein features. Potential functions could involve modulating host's intracellular trafficking processes, particularly during initial stages of infection.

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Isolation and Characterisation of the Bundooravirus Genus and Phylogenetic Investigation of the Salasmaviridae Bacteriophages

https://www.mdpi.com/1999-4915/13/8/1557/htm

Orf18 closest homologues are found in other phi29-like phages WhyPhy and SRT01hs and a Staphylococcus phage ST134 with a conserved motif TIGR04523. This is followed by another morphogenesis protein, orf19, with the characteristic motif pfam01551.