As promised in my earlier post about sterilizing tomato seed below I show how I prepare media for tissue culture purposes. In this case I am germinating tomato seed in an effort to end up with sterile plant material to work with in vitro. The media is called standard M&S mixtrophic mixture or MSO. M stands for Murashige and S stands for Skoog. A number behind the letters MS is used to indicate the sucrose concentration of the medium. For example, MS0 contains no sucrose and MS20 contains 20 g/l sucrose.
MSO was invented by plant scientists Toshio Murashige and Folke K. Skoog in 1962 during Murashige's search for a new plant growth regulator.
Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators
