"To detect BNT162b2 RNA level, we designed primers with forward primer located in SARS-CoV-2 S protein regions and reverse primer in 30 -UTR, which allows detection of PCR amplicon unique to BNT162b2 without unspecific binding of the primers to human genomic regions",

"RT-qPCR results showed that Huh7 cells treated with BNT162b2 had high levels of BNT162b2 mRNA relative to housekeeping genes at 6, 24, and 48 h (Figure 2, presented in logged 2−∆∆CT due to exceptionally high levels).

The three BNT162b2 concentrations led to similar intracellular BNT162b2 mRNA levels at the different time points, except that the significant difference between 1.0 and 2.0 µg/mL was observed at 48 h.

BNT162b2 mRNA levels were significantly decreased at 24 h compared to 6 h, but increased again at 48 h.", -p5 of PDF 1119 in doc,

[ using the method described in #34 (Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line https://www.mdpi.com/1467-3045/44/3/73) "The full sequence of BNT162b2 is publicly available [34] and contains a two-nucleotides cap;50 - untranslated region (UTR) that incorporates the 50 -UTR of a human α-globin gene;" ]