Cold-Stored Mycelial Transfer - Petri, Agar, and Transfer Methodology

Welcome to my summary of this weeks work on petri dish utilization via transfer and isolation of cold-stored psilocybin multi-strained dishes!

This may sound confusing, but, simply put, there were three days of work and essentially three major steps to this process:

  • Clean, Sanitize, and Sterilize Dishes
  • Prepare and Pour Agar Agar, and
  • Cut cold-stored Petri storage colonies to these newly prepared Agar Petri Dishes.

So, without further adieu, let's begin!

Wednesday, January 12th 2022


This day was spent preparing my dishes for cleaning.

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After receiving,

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I prepared my work station,

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With utilization of Clorox after a soap and water clean and rinse for both 2 minutes, 99% Isopropyl Alcohol is used to soak the dish for 15 seconds.

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The dishes cleaned well, and were ready after a nights dry to be used tomorrow!

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Yay! Past Section 1.1.1 and 2 of my tek, this is seen in Section 1.2: - @trezzahn/mycelial-spore-utilization-on-agar-petri-dishes-p2-4-in-a-two-blog-series

Thursday, January 13th 2022


Onward! Today I prepared Agar! I used my reagent ingredient recipe found here - @trezzahn/an-introduction-to-agar-and-its-utilization-working-with-it-proper-technique-and-further-use-p2-4-in-a-two-blog-series
And following proper procedure, stove-top cooked my Agar Agar recipe to create Agar -

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Such for use pouring in my own now dried Petri dishes -

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And letting these now poured Agar dishes cool overnight until the following day!

Friday, January 14th 2022


Lastly, these now poured Agar Dishes are cooled and ready for use!

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These are prepared for use transferring previously cold-stored Petri's to these new dishes - something done every 6 months to ensure viability -

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And as these prepared dishes are cut,

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according to my tek Section 1.3.2 found here - @trezzahn/mycelium-growth-preparation-agar-additionals-general-timeframes-and-further-work-the-fun-part
I transfer, beautifully, two Golden Mammoth - Ajax, two Golden Capped - Penis Envy and One P. Cyanescens to these five prepared Agar Petri's;

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Concluding my three day journey this week working with psilocybin mycelium on Agar Petri Dishes, I ask the question.

What has everybody else been up to this week?

Stay safe, stay somber!

Update: See next weeks work here @trezzahn/cold-stored-mycelial-transfer-part-two-petri-agar-and-transfer-methodology--examination-of-last-weeks-work

Trezzahn



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@trezzahn/blog

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