Cold-Stored Mycelial Transfer: Part Two - Petri, Agar, and Transfer Methodology + Examination of Last Week's Work

Welcome to my summary of this weeks work on some new petri dish utilization via transfer and isolation of cold-stored psilocybin multi-strained dishes, as well as an examination of last weeks cold-stored mycelial transfers!


This may sound confusing, but, simply put, there were three days of work and essentially three major steps to this process, similar to last weeks, found here @trezzahn/cold-stored-mycelial-transfer-petri-agar-and-transfer-methodology or here

  • Clean, Sanitize, and Sterilize Dishes,
  • Prepare and Pour Agar Agar, and
  • Cut cold-stored Petri storage colonies to these newly prepared Agar Petri Dishes.

So, without further adieu, let's begin examination!

Monday January 17th - Wednesday January 19th, 2022

These days were spent examining the growth of psilocybin mycelium on agar petri dishes;




Effective growth is evident in four of five dishes, as will be showcased in the following days.


Thursday, January 20th 2022

This day was spent preparing my dishes for cleaning.


I prepared my work station, and

With utilization of Clorox after a soap and water clean and rinse for both 2 minutes, 99% Isopropyl Alcohol was used to soak the dishes for 15 seconds.

The dishes cleaned well, and were ready after a nights dry to be used Friday!


Now past Section 1.1.1 and 2 of my tek, seen in Section 1.2: - @trezzahn/mycelial-spore-utilization-on-agar-petri-dishes-p2-4-in-a-two-blog-series

Cleaning complete!

Friday, January 21st 2022

Onward! Today I prepared Agar!


I used my reagent ingredient recipe found here - @trezzahn/an-introduction-to-agar-and-its-utilization-working-with-it-proper-technique-and-further-use-p2-4-in-a-two-blog-series


And following proper procedure, stove-top cooked my Agar Agar recipe to create Agar -


Ensuring proper pressure cooking could be complete;


And letting these now poured Agar dishes cool overnight until the following day!


Pouring complete!

Saturday, January 22nd 2022

Lastly, after examination of previous dishes,


These poured Agar Dishes from yesterday are cooled and ready for use!


These are prepared for use transferring previously cold-stored Petri's to these new dishes - something done every 6 months to ensure viability -


And as these prepared dishes are cut according to my tek Section 1.3.2 found here - @trezzahn/mycelium-growth-preparation-agar-additionals-general-timeframes-and-further-work-the-fun-part
I transfer, beautifully, three Golden Mammoth - Ajax, and two Golden Capped - Penis Envy to these five prepared Agar Petri's;


Transfers complete!

Sunday, January 23rd 2022

This final day, I examined the progress of my Petri's from last week, below;


And yesterday's transfers, with no progress visible yet but most likely to begin colonizing by end day, below;


Ultimately showcasing today's work and the progress of all dishes colonizing!

Ultimately, thus, concluding my three day journey this week and seven day adventure of colonization working with psilocybin mycelium on Agar Petri Dishes.

Now, again, I ask the question.

What has everybody else been up to this week?

Stay safe, stay somber!


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